Modifying Enzymes
Worthington Biochemical Corporation WORTHINGTON BIOCHEMICAL CORPORATION
NC4031143 RIBONUCLEASE T2 RECOMBINANT 50
AURORA BIOLABS LLC Enterokinase (EK) Activity Assay Kit,96 rxn
The Enterokinase (EK) Activity Assay Kit is a fluorogenic assay designed to quantitatively measure EK protease activity. The kit includes a specific EK substrate (Asp-Asp-Asp-Asp-Lys) conjugated to the fluorophore AFC (7-amino-4-trifluoromethylcoumarin). Upon proteolytic cleavage of the substrate by EK, free AFC is released, generating a strong fluorescent signal. The fluorescence can be detected using a microplate fluorescence reader with excitation at 365 nm and emission at 505 nm. The increase in fluorescence intensity is directly proportional to EK enzymatic activity and can be used to calculate protease activity. Purified EK protease is provided in the kit as a positive control to validate assay performance and ensure proper assay setup.
INTACT GENOMICS INC Taq DNA Ligase, 2,000 Units (50 µl)
Taq DNA Ligase provides high specificity for ligation-based PCR, SNP detection, and molecular diagnostics. This enzyme catalyzes phosphodiester bond formation in duplex DNA, using NAD+. It enables precise, high-temperature ligation (45°C to 70°C), suitable for applications requiring accuracy, including single-base substitution detection.
- High specificity for PCR, SNP detection, and molecular diagnostics.
- Enables precise, high-temperature DNA ligation (45°C to 70°C).
- Catalyzes phosphodiester bond formation in duplex DNA with NAD+ cofactor.
- Detects single-base substitutions; protein purity ≥99%.
- Free from detectable RNase and DNA endonuclease activities.
- Ideal for allele-specific gene detection (LDR, LCR) and mutagenesis.
- Includes Taq DNA Ligase and 10X Taq DNA Ligase Buffer.
INTACT GENOMICS INC T7 Endonuclease I, 1,250 Units
T7 Endonuclease I is a stable homodimer of identical 149 amino acid subunits, produced from a recombinant gene in *E. coli*. It recognizes and cleaves mismatched DNA where the mismatched DNA length position is more than 1 bp. This enzyme is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells, which are generated by CRISPR/ZFN/TALEN at desired target sites, PCR-amplified, denatured, and re-annealed to form mismatched DNA.
- Resolves four-way junction or branched DNA.
- Detects or cleaves heteroduplex and nicked DNA.
- Randomly cleaves linear DNA for shot-gun cloning.
- Physical purity is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining.
- Includes T7 Endonuclease I and 10x T7 Endonuclease I reaction buffer.
- Storage temperature: -20°C.
- Free from detectable contaminating nuclease activities.
INTACT GENOMICS INC Tth DNA Ligase, 10,000 units
High-fidelity Thermus thermophilus (Tth) DNA Ligase from Intact Genomics delivers precise, thermostable ligation with excellent SNP discrimination and low background—ideal for LCR and mutation detection.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC3999297 ENTEROKINASE ENTEROPEPTIDASE
American Research Products Inc LIPASE KIT COLORIMETRIC BA0052
5000265510 LIPASE KIT COLORIMETRIC BA0052
American Research Products Inc CELLULASE CL COLORIMETRIC KIT
5000265552 CELLULASE CL COLORIMETRIC KIT