Modifying Enzymes
New England Biolabs, Inc. AluI Methyltransferase – 100 units
AluI Methyltransferase modifies the cytosine residue (C5) of the sequence AGCT.
New England Biolabs, Inc. α1-2 Fucosidase - 1000 units
a1-2 Fucosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of linear 1-2 linked fucose residues from oligosaccharides
New England Biolabs, Inc. Uracil-DNA Glycosylase (UDG) – 5000 units
E. coli Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).
Sigma Aldrich Fine Chemicals Biosciences Malonyl 13C3 coenzyme A li10MG
Malonyl 13C3 coenzyme A li10MG
Amsbio LLC Chondroitinase AC-I (EC 4.2.2.5) from Flavobacterium heparinum/ 20 IU
Chondroitinase AC-I (EC 4.2.2.5) from Flavobacterium heparinum 20 IU
Amsbio LLC Chondroitinase B from Flavobacterium heparinum/ 2 IU
Chondroitinase B from Flavobacterium heparinum 2 IU
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES ANTI-TOPOISOMERASE I-DNA COVAL
NC3728455 ANTI-TOPOISOMERASE I-DNA COVAL
Ubpbio HUMAN UBE1, 25UG
E1 Activating Enzymes – UbE1 (UBPBio catalog# B1100, B1101) – Purified from sf9 insect cells. UBE1 is an E1 enzyme within the E1, E2, E3 cascade which conjugates Ub to protein substrates. UBE1 activates Ub by catalyzing an ATP – dependent reaction; Ub is conjugated onto the catalytic Cys residue of UBE1 by formation of a thioester bond. The activated Ub is then passed onto a Ub-conjugating enzyme E2; Ub charged E2 binds a Ub ligase E3 that directly binds substrate proteins. Ub is transferred to the HECT domain E3s prior to being conjugated on substrate proteins. For RING domain E3s, Ub is transferred directly from an E2 to substrate proteins.
New England Biolabs, Inc. Thermostable 5' App DNA/RNA Ligase – 10 reactions
Thermostable 5' App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum. This enzyme is ATP independent. It requires a 5' pre-adenylated linker for ligation to the 3' -OH end of either RNA or single stranded DNA (ssDNA). The enzyme is also active in ligation of RNA with 2' -O-methylated 3 end to 5-adenylated linkers. The optimal temperature for ligation reaction is 60-65°C. The mutant ligase is unable to adenylate the 5' -phosphate of RNA or ssDNA, which reduces the formation of undesired ligation products (concatemers and circles). The ability of the ligase to function at 65°C might reduce the constraints of RNA secondary structure in RNA ligation experiments.
Medchemexpress LLC Lipase from Pseudomonas fluorescens (immobilized) | 9001-62-1 | MFCD00131509 | 1g
Lipase Pseudomonas fluorescens (Immobilized) comes from Pseudomonas fluorescens and shows good applicability in ester synthesis of food industry the biofuel synthesis the synthesis of enantiomeric pure chiral drugs and so on[1]
Worthington Biochemical Corporation HYALURONIDASE PURIFIED 5KUHYA
Chromatographically purified. A dialyzed, lyophilized powder.